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Chromatin immunoprecipitation to reveal the location of transcription factor TFIIS binding in yeast

ABSTRACT: Genome-wide location of transcription factor TFIIS

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yad Ghavi-helm 

PROVIDER: E-MTAB-10 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide location analysis reveals a role of TFIIS in RNA polymerase III transcription.

Ghavi-Helm Yad Y   Michaut Magali M   Acker Joël J   Aude Jean-Christophe JC   Thuriaux Pierre P   Werner Michel M   Soutourina Julie J  

Genes & development 20080701 14

TFIIS is a transcription elongation factor that stimulates transcript cleavage activity of arrested RNA polymerase II (Pol II). Recent studies revealed that TFIIS has also a role in Pol II transcription initiation. To improve our understanding of TFIIS function in vivo, we performed genome-wide location analysis of this factor. Under normal growth conditions, TFIIS was detected on Pol II-transcribed genes, and TFIIS occupancy was well correlated with that of Pol II, indicating that TFIIS recruit  ...[more]

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