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RNA-seq of mouse ileum to investigate the role of IL-17 receptor signaling in Paneth cells in their biology and intestinal physiology

ABSTRACT: IL-17 receptor plays a major role in the antibacterial defense and regulation of host-microbiota interaction. However its impact on Paneth cells, cell-type specific for production of antibacterial peptides, is still not clear. Here, we used genetic depletion of IL-17 receptor specific for Paneth cell population (Defa6-iCre x Il17ra-flox) and performed the RNA sequencing on FACS-sorted Paneth cells and also complete ileum tissue, comparing Cre+ and Cre- littermates.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Dominik Filipp

PROVIDER: E-MTAB-10038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-17-driven induction of Paneth cell antimicrobial functions protects the host from microbiota dysbiosis and inflammation in the ileum.

Brabec Tomáš T Vobořil Matouš M Schierová Dagmar D Valter Evgeny E Šplíchalová Iva I Dobeš Jan J Březina Jiří J Dobešová Martina M Aidarova Aigerim A Jakubec Martin M Manning Jasper J Blumberg Richard R Waisman Ari A Kolář Michal M Kubovčiak Jan J Šrůtková Dagmar D Hudcovic Tomáš T Schwarzer Martin M Froňková Eva E Pinkasová Tereza T Jabandžiev Petr P Filipp Dominik D

Mucosal immunology 20230203 4

Interleukin (IL)-17 protects epithelial barriers by inducing the secretion of antimicrobial peptides. However, the effect of IL-17 on Paneth cells (PCs), the major producers of antimicrobial peptides in the small intestine, is unclear. Here, we show that the targeted ablation of the IL-17 receptor (IL-17R) in PCs disrupts their antimicrobial functions and decreases the frequency of ileal PCs. These changes become more pronounced after colonization with IL-17 inducing segmented filamentous bacter ...[more]

PMID: 36739089

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