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MicroRNA profiling of Draining Lymph node (popliteal and inguinal) from mice with delayed hypersensitivity treated with vehicle control, TCDD and FICZ

ABSTRACT: Aryl hydrocarbon receptor (AhR), is a transcription factor and an environmental sensor and AhR ligands exert varying effects from suppression to exacerbation of inflammation, the reason for which is unclear. In the current study, we demonstrate for the first time that the differential effects of AhR ligands on T cell differentiation (Tregs vs Th-17 cells) is mediated by miRNA, specifically, miRNA-132. We also demonstrate that miRNA-132 targets High Mobility Group Box 1 (HMGB1), which in turn regulates the differentiation of FoxP3+ Tregs. We demonstrate the role of miRNA-132 conclusively using transfection studies and the use of mice deficient in miRNA-132. We wish to point out that our studies are novel in that the role of HMGB1 in the regulation of Tregs has not been previously reported. To this end, we used popliteal and inguinal lymph nodes (DLNs) of mice (C57BL/6) with DTH and treated with Vehicle (corn oil, VEH) or TCDD or FICZ. In brief, total RNA including miRs from DLNs were isolated using miRNeasy kit and the protocol of the company was followed (QIAGEN, Valencia, CA). Next, miR arrays were performed at Johns Hopkins University Microarray and Sequencing Core facility using platform.

ORGANISM(S): Mus musculus

SUBMITTER: Osama Abdulla

PROVIDER: E-MTAB-10117 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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