Project description:Candida albicans strains SC5314 and YJB-T1891 were used as wild type to delete VMA11. The deletion strains (TJ926 and TJ927) were spread on YPD plate supplemented with 1μg/ml fluconazole. Randomly 96 adaptors derived from TJ926 and 96 adaptors derived from TJ927 were tested with disk diffusion assay. Among the resistant adaptors, randomly 15 adaptors (A4-A18) and 48 adaptors (A19-A66) derived from TJ926 were sequenced.
Project description:Candida auris clinical isolate FY279 was exposed to tebuconazole (32μg/ml). Randomly14 adaptors were chosen. 10 adaptors obtained resistance to tebuconazole. These resistant adaptors were sequenced.
Project description:Candida auris clade III isolate B12039 was spread on YPD plate supplemented with 250 ng/ml, 500 ng/ml, 1000 ng/ml, and 2000 ng/ml of caspofungin. In total, 33 adaptors were chosen for further analysis. We did sequencing of them as as well as the parent. 9 adaptors (250-1 - 250-9) were obtained from YPD+250ng/ml caspofugin. 6 adaptors (500-1 - 500-6) were obtained from YPD+250ng/ml caspofugin. 6 adaptors (1000-1 - 1000-6) were obtained from YPD+250ng/ml caspofugin. 12 adaptors (2000-1 - 2000-12) were obtained from YPD+250ng/ml caspofugin
Project description:YJB-T490 was spread on YPD-agar plates supplemented with miconazole (0.008-2ug/mL). Randomly 3 adaptors from each drug plate were sequenced. In addition, 2 miconazole adaptors derived from SC5314, and 6 adaptors derived from CDR1 deletion strain were also sequenced.
Project description:Candida albicans lab strain SC53114 was used to obtain fluconazole-tolerant adaptors. 53 adaptors as well as the parent strain were sequenced.
Project description:Candida parapsilosis chromosome 1 trisomy strain TJ74 was exposed to 400ng/ml caspofungin. Randomly 30 adaptors were chosen. These adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 1.5ug/ml pyraclostrobin. Randomly 23 adaptors were chosen. These adaptors were sequenced.
Project description:We used Candida albicans lab strain SC5314 to obtain tunicamycin adaptors. We did whole genome sequencing of the adaptors and the parent as well.
Project description:Cryptococcus neoformans lab strain H99 was exposed to 600ng/ml aureobasidin A. Randomly 30 adaptors were chosen. These adaptors and the wild type strains were sequenced.
Project description:Candida parapsilosis wild type strain #12108 was exposed to 400ng/ml caspofungin. Randomly 30 adaptors were chosen. These adaptors as well as the parent were sequenced.