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3’RNA-seq of murine post-differentiation CD142− ASPCs treated with retinoic acid (RA)

ABSTRACT: To study the impact of retinoic acid treatment on the transcriptome of CD142− ASPCs, we performed bulk RNA-seq on CD142− ASPCs treated with different RA concentrations ranging between 0.01 to 100 μM, with 100 ng/ml of BSA (negative control), DMSO (negative control) or 100 ng/ml of EGF (positive control).

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Pernille Rainer

PROVIDER: E-MTAB-10184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mammalian adipogenesis regulator (Areg) cells use retinoic acid signalling to be non- and anti-adipogenic in age-dependent manner.

Zachara Magda M Rainer Pernille Y PY Hashimi Horia H Russeil Julie M JM Alpern Daniel D Ferrero Radiana R Litovchenko Maria M Deplancke Bart B

The EMBO journal 20220822 18

Adipose stem and precursor cells (ASPCs) give rise to adipocytes and determine the composition and plasticity of adipose tissue. Recently, several studies have demonstrated that ASPCs partition into at least three distinct cell subpopulations, including the enigmatic CD142<sup>+</sup> cells. An outstanding challenge is to functionally characterise this population, as discrepant properties, from adipogenic to non- and anti-adipogenic, have been reported for these cells. To resolve these phenotypi ...[more]

PMID: 35996853

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