Project description:Transcriptional profiling of neuroblastoma cell line expressing the PML1 isoform. Two-condition experiment: PML1 IMR32 vs empty vector IMR32. Two biological replicates, dye-swapped.
Project description:IMR32 was transduced with control or shMYCN virus (duplo). For each experiment 3 time points were analyzed and 2 no virus controls. IMR32 was transduced with control or shMYCN virus (duplo). For each experiment 3 time points were analyzed and 2 no virus controls.
Project description:Cells were cultured in RPMI 1640 (Gibco), supplemented with 10% fetal bovine serum (FBS) (Gibco), 2mM L-glutamine (Gibco) and 1% Penicillin-Streptomycin solution (Gibco). IMR32 cells were treated as follows; untreated control, 24h 1uM azakenpaullone, 24h 1uM BIO or 24h 28mM LiCl with biological duplicates.