Unknown,Transcriptomics,Genomics,Proteomics

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Identification of ETO2 direct target genes through integration of transcriptomic (RNAseq) and chromatin binding location (ChIPseq)


ABSTRACT: Erythroid cell lines (HEL and K562) were conditionally invalidated for the ETO2 gene using the CRISPR/Cas9 system. Gene expression profiling (RNAseq) and chromatin immunoprecipitation followed by high-throughput sequencing (ChIPseq) to assess localization of ETO2, MYB, EP300, H3K27ac and H3K4me3 was performed in control and ETO2-deficient cells. ChIPseq analyses were also performed on cells from human AEL patient-derived xenograft models.

INSTRUMENT(S): Illumina HiSeq 4000, Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Alexandre Fagnan 

PROVIDER: E-MTAB-10346 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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