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SnATAC-seq of the gray short-tailed opossum cerebellum in two developmental stages

ABSTRACT: This study used droplet-based snATAC-seq to profile the chromatin accessibility landscape of 19,204 nuclei in the opossum (Monodelphis domestical) cerebellum across two developmental stages (postnatal day 21 and adult). The study included two biological replicates per stage, one from each sex, and an additional adult sample enriched for white matter. Cerebelli were dissected in two halves, nuclei were extracted from one half and profiled using 10x single-cell ATAC reagent kit (v1.1) and a Chromium controller. The white matter enriched sample was dissected from coronal cerebellum slices. Libraries were sequenced using paired-reads on Illumina NextSeq 550 and initial data processing was performed using Cellranger ATAC (1.1).

INSTRUMENT(S): Chromium 10x, NextSeq 550

ORGANISM(S): Monodelphis domestica

SUBMITTER: Ioannis Sarropoulos 

PROVIDER: E-MTAB-10533 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Organ development is orchestrated by cell- and time-specific gene regulatory networks. In this study, we investigated the regulatory basis of mouse cerebellum development from early neurogenesis to adulthood. By acquiring snATAC-seq (single-nucleus assay for transposase accessible chromatin using sequencing) profiles for ~90,000 cells spanning 11 stages, we mapped cerebellar cell types and identified candidate cis<i>-</i>regulatory elements (CREs). We detected extensive spatiotemporal heterogene  ...[more]

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