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SnATAC-seq of the developing mouse cerebellum across eleven stages

ABSTRACT: This study used droplet-based snATAC-seq to profile the chromatin accessibility landscape of 91,922 nuclei in the mouse cerebellum across eleven developmental stages, from the beginning of neurogenesis (e10.5) till adulthood (P63). The study included two biological replicates per stage, one from each sex. Cerebelli were dissected as whole or in two halves, nuclei were extracted and profiled using 10x single-cell ATAC reagent kit (v1.0) and a Chromium controller. Libraries were sequenced using paired-reads on Illumina NextSeq 550 and initial data processing was performed using Cellranger ATAC (1.1).

INSTRUMENT(S): Chromium 10x, NextSeq 550

ORGANISM(S): Mus musculus

SUBMITTER: Ioannis Sarropoulos 

PROVIDER: E-MTAB-9765 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Organ development is orchestrated by cell- and time-specific gene regulatory networks. In this study, we investigated the regulatory basis of mouse cerebellum development from early neurogenesis to adulthood. By acquiring snATAC-seq (single-nucleus assay for transposase accessible chromatin using sequencing) profiles for ~90,000 cells spanning 11 stages, we mapped cerebellar cell types and identified candidate cis<i>-</i>regulatory elements (CREs). We detected extensive spatiotemporal heterogene  ...[more]

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