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Time-coursed transcriptomics of ATRA-induced granulocytic differentiation

ABSTRACT: Transcriptional profiling of HL-60 cells at 0, 3, 24, and 96 h after ATRA treatment. The differentially expressed genes was meant to be the input data for bioinformatic upstream regulator search

ORGANISM(S): Homo sapiens

SUBMITTER: Svetlana Novikova

PROVIDER: E-MTAB-10606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomics of HL60 cell line at 30 min and 1 h after all-trans-retinoic acid (ATRA) treatment

Project description:Transcriptome profiling of HL60 cells at the yearly time points of ATRA treatment (30 min and 1 h) allows to unveil molecular mechanisms of the onset of granulocytic maturation. Transcriptomic techniques are characterized by high sensitivity and allow for detection of low-abundant molecules, such as nuclear transcription factors.

2022-10-01 | E-MTAB-12026 | biostudies-arrayexpress

Nuclear proteomics of induced granulocytic differentiation

Project description:The nuclear proteome harbors a sheer number of regulatory proteins, including transcription factors (TFs). Profiling of nuclear proteome during all-trans-retinoid acid(ATRA)-induced differentiation of HL60 cells allows to unveil molecular mechanisms of granulocytic maturation. It is especially important to have an understanding of molecular perturbations at the early stages of the differentiation process. Applying proteomic profiling using isobaric labeling coupled with alkaline fractionation (TMT/2D) we identified 1860 nuclear proteins with high confidence (FDR<0.01, at least 2 unique peptides per protein). Among them 136, 226, 280, 312 and 241 proteins were found to be altered at 3, 6, 9, 12, and 72 h in HL60 cell nuclear fraction under ATRA treatment.

2023-03-11 | PXD035281 | Pride

Transcription profiling of human HL-60 Cells Stimulated by ATRA and DMSO

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Transcriptome dynamics of HL-60, NB4, and K562 cells under the ATRA treatment

Project description:Combination of new therapeutics with trans-retinoic acid (ATRA) could improve efficiency of acute myeloid leukemia (AML) treatment. Modeling the process of ATRA-induced differentiation based on transcriptomic profile of leukemic cells allows us to identify key molecules that could be affected to enhance the therapeutic effect of ATRA. Moreover, transcriptome analysis reveals the earliest steps of molecular response to inducer treatment. Thus, the transcriptomic profile of leukemic cells under the ATRA treatment at the different time points is considered as input data for further upstream regulator search.

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Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia [Illumina HumanHT-12 gene expression array]

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2017-11-09 | PXD006421 | Pride

Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia

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Transcription profiling of human epithelial cell line (HL) infected with Chlamydia pneumoniae

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2008-06-15 | E-GEOD-7246 | biostudies-arrayexpress

The Study of ATRA-Induced Differentiation of the HL-60 Cell Line

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2021-09-07 | PXD006768 | Pride

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