Dataset Information

Nuclear proteomics of induced granulocytic differentiation

ABSTRACT: The nuclear proteome harbors a sheer number of regulatory proteins, including transcription factors (TFs). Profiling of nuclear proteome during all-trans-retinoid acid(ATRA)-induced differentiation of HL60 cells allows to unveil molecular mechanisms of granulocytic maturation. It is especially important to have an understanding of molecular perturbations at the early stages of the differentiation process. Applying proteomic profiling using isobaric labeling coupled with alkaline fractionation (TMT/2D) we identified 1860 nuclear proteins with high confidence (FDR<0.01, at least 2 unique peptides per protein). Among them 136, 226, 280, 312 and 241 proteins were found to be altered at 3, 6, 9, 12, and 72 h in HL60 cell nuclear fraction under ATRA treatment.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Svetlana Novikova

LAB HEAD: Victor Zgoda

PROVIDER: PXD035281 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	110322RNF_NSE_2DTMT_Nucl_L1F01.raw	Raw
	110322RNF_NSE_2DTMT_Nucl_L1F01_20220313053457.raw	Raw
	110322RNF_NSE_2DTMT_Nucl_L1F01_20220313071841.raw	Raw
	110322RNF_NSE_2DTMT_Nucl_L1F02.raw	Raw
	110322RNF_NSE_2DTMT_Nucl_L1F02_20220313104613.raw	Raw

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Publications

Nuclear Proteomics of Induced Leukemia Cell Differentiation.

Novikova Svetlana S Tolstova Tatiana T Kurbatov Leonid L Farafonova Tatiana T Tikhonova Olga O Soloveva Natalia N Rusanov Alexander A Archakov Alexander A Zgoda Victor V

Cells 20221014 20

Studies of induced granulocytic differentiation help to reveal molecular mechanisms of cell maturation. The nuclear proteome represents a rich source of regulatory molecules, including transcription factors (TFs). It is important to have an understanding of molecular perturbations at the early stages of the differentiation processes. By applying the proteomic quantitative profiling using isobaric labeling, we found that the contents of 214, 319, 376, 426, and 391 proteins were altered at 3, 6, 9 ...[more]

PMID: 36291090

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