Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

91 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of CT26 cells expressing control or Gna13 targeting sgRNAs

ABSTRACT: CT26 cells expressing lentiviral Cas9 and sgRNAs targeting either control or Gna13

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Martin

PROVIDER: E-MTAB-10727 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of CT26 subcutaneous tumors expressing control or Gna13 targeting sgRNAs

Project description:CT26 cells expressing lentiviral Cas9 and sgRNAs targeting either control or Gna13 were transplanted into immunocompetent BALB/c mice. Tumors were harvested and processed for RNA-seq

2021-10-01 | E-MTAB-10737 | biostudies-arrayexpress

RNA-seq of CT26 cells expressing TRE-GNA13 Q226L

Project description:CT26 cells expressing a lentiviral TRE-GNA13 Q226L transgene were untreated or treated with 100 ng/mL doxycycline for 48h

2021-10-01 | E-MTAB-10726 | biostudies-arrayexpress

RNA-seq of CT26 cells expressing control or Gna13 targeting sgRNAs

Project description:RNA-seq of CT26 cells expressing control or Gna13 targeting sgRNAs

| PRJEB46347 | ENA

RNA-seq of CT26 subcutaneous tumors expressing control or Gna13 targeting sgRNAs

Project description:RNA-seq of CT26 subcutaneous tumors expressing control or Gna13 targeting sgRNAs

| PRJEB46348 | ENA

Targeted DNA-seq of Human HepG2 cells following editing with CRISPR/Cas9

Project description:We performed a large-scale genome-wide characterisation of indels generated following editing with CRISPR/Cas9. We used pools of sgRNAs and performed targeted capture and sequencing of the edited regions in HepG2 cells.

2018-12-13 | E-MTAB-7095 | biostudies-arrayexpress

CT26 RNA-Seq

Project description:RNA-Seq of CT26 mouse colorectal carcinoma and gene expression profiling

2014-02-07 | E-MTAB-2292 | biostudies-arrayexpress

Generation of LAMC2-EGFP knock-in human pancreatic cancer cells

Project description:Since LAMC2 is a secreted molecule present in the extracellular matrix of the cells, was designed a strategy based on CRISPR/Cas9-mediated homologous recombination to mark LAMC2 cells in human PDACs. A Cas9 single-guide RNAs complementary to sequences overlapping the stop codon of the LAMC2 locus was designed and a donor vector that contained LAMC2 homology arms flanking an EGFP reporter cassette positioned immediately upstream of the stop codon was generated. LF2A self-cleavage peptide in frame with EGFP so that LAMC2-EGFP locus was expressed as a single mRNA was added, whereas the resulting polypeptide was cleaved in the two encoded proteins, LAMC2 and EGFP. L3.6pl and PANC-1 cells were nucleofected with the donor vector together with a guide-RNA-Cas9 (guide). The engineered cells were subcutaneously injected in CD1 male mice and RNA-seq was performed on LAMC2-EGFP+ and EGFP- -derived tumors.

2022-04-11 | E-MTAB-11597 | biostudies-arrayexpress

RNA-seq data of rs2280381-containing region knock out clone in U-937 cell line

Project description:rs2280381 is an autoimmune disease susceptible variant, which has been reported to be associated with SLE, RA and SSc. Genetic and epigenomic analysis indicate that the rs2280381-containing region is an enhancer region in monocyte. To test the function and regulated genes of the rs228381-containing region, we deleted the rs2280381 ~120bp sequence in U-937 cells, and performed the RNA-seq to detect the gene expression profiles.

2022-02-25 | E-MTAB-11306 | biostudies-arrayexpress

RNA seq in U-937 cells wildtype clones and rs2431697 region deletion clones

Project description:rs2431697 is a SLE risk SNP, genetic and epigenomic analysis indicate that rs2431697-containing region is an enhancer region. To test the function and regulated genes of rs2431697-containing genes, we deleted rs2431697-containing region in U-937 cells, and performed the RNA-seq to detect the gene expression profiles.

2020-11-05 | E-MTAB-8978 | biostudies-arrayexpress

RNA-seq of rs2280381-containing region knock out clone in U937

Project description:rs2280381 is an autoimmune disease susceptible loci, which have be reported to be associated with SLE, RA and SSc.Genetic and epigenomic analysis indicate that rs2280381-containing region is an enhancer region in monocyte. To test the function and regulated genes of rs228381-containing region, we deleted rs2280381 ~120bp sequence in U-937 cells, and performed the RNA-seq to detect the gene expression profiles.

2021-07-01 | E-MTAB-10126 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data