Project description:OrR drosophila 3rd instar larvae were subjected to septic injury with a mixture of E.coli and S.aureus at 3h, 6h and 18h. Plasmatocytes were isolated afterwards and subjected to RNA-seq
Project description:Drosophila carrying Hml-SwitchGal4 were crossed to different uas-constructs (uas-eGFP, uas-PGRP-LC, uas-hop, uas-tkvQD). 3rd instar larvae were either kept as control or SwitchGal4 was activated by hormone feeding for 24h. Afterwards plasmatocytes were extracted and subjected to RNA-seq.
Project description:Drosophila plasmatocyes expressed control or trxG RNAis under the plasmatocyte specific Hml promotor. 6h prior to plasmatocyte collection animals were split into control and septic injury groups and treated accordingly. Afterwards plasmatocytes were isolated and polyA RNA was subjected to RNA-seq.
Project description:Drosophila 3rd instar larvae were subjected to septic injury. After 6h plasmatocytes were isolated, fixed and subjected to ChIP-seq.
Project description:To identify the presence of different chromatin states in homogeneous primary cells, Drosophila melanogaster plasmatocytes were isolated from 3rd instar larvae and subjected to cross-linked ChIP-seq using antibodies against a range of H3 histone modifications and PolII.
Project description:Drosophila melanogaster 3rd instar wandering larvae with a genetic construct for hemocyte ablation were collected along with genetically matched non-ablated animals, RNA was extracted from whole animals and used for RNA-seq.
Project description:mRNA expression levels were determined by NGS for wildtype larvae as well as for larvae lacking HP1a [Su(var)205^04/Su(var)205^05 transheterozygotes]. RNA samples from wildtype (OR) and HP1a mutant third instar larvae were examined, using duplicate biological samples and Illumina NGS.
Project description:The Hippo pathway regulates metazoan growth, acting through the transcriptional co-activators Yorkie (in Drosophila) and Yap and Taz (in vertebrates). Much attention has been focused on upstream regulators of Yorkie and its homologues. In contrast, the mechanisms by which they actually promote transcription have remained poorly understood. Genome-wide chromatin binding experiments support extensive functional overlap between Yorkie and GAF. Chromatin binding identifies thousands of Yorkie sites, the majority of which are associated with elevated transcription, based on genome-wide analysis of mRNA and histone H3K4Me3 modification. Our studies establish a molecular basis for transcriptional activation by Yorkie and implicate it as a global regulator of transcriptional activity in Drosophila. This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. This dataset was generated in collaboration with Ken Irvine at HHMI/Rutgers University and Richard S. Mann at Columbia University. It contains RNA-seq data for larval wing imaginal discs.