Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of total and nascent RNA after depletion of Mpe1


ABSTRACT: To test the role of Mpe1 in transcription termination we inserted a mini-auxin induced degron (mAID) at the C-terminal end of the endogenous MPE1 locus in the yeast Saccharomyces cerevisiae. We treated Mpe1-mAID cells (JRY101) or wild type cells (WT, YMK728) with 1 mM auxin for 30 minutes and labeled the nascent RNA with 4-thiouracil (4tU) for 6 minutes. The nascent RNA fraction was prepared by biotinylating the 4tU-labeled RNA followed purification with streptavidin beads. Nascent and total RNA fractions were depleted of ribosomal RNA and strand-specific libraries prepared. Libraries were single-end sequenced using an Ilummina HiSeq 4000 instrument.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Saccharomyces cerevisiae S288c

SUBMITTER: Juan Rodriguez-Molina 

PROVIDER: E-MTAB-10820 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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