RNA-seq of total and nascent RNA after depletion of Mpe1
Ontology highlight
ABSTRACT: To test the role of Mpe1 in transcription termination we inserted a mini-auxin induced degron (mAID) at the C-terminal end of the endogenous MPE1 locus in the yeast Saccharomyces cerevisiae. We treated Mpe1-mAID cells (JRY101) or wild type cells (WT, YMK728) with 1 mM auxin for 30 minutes and labeled the nascent RNA with 4-thiouracil (4tU) for 6 minutes. The nascent RNA fraction was prepared by biotinylating the 4tU-labeled RNA followed purification with streptavidin beads. Nascent and total RNA fractions were depleted of ribosomal RNA and strand-specific libraries prepared. Libraries were single-end sequenced using an Ilummina HiSeq 4000 instrument.
INSTRUMENT(S): Illumina HiSeq 4000
ORGANISM(S): Saccharomyces cerevisiae S288c
SUBMITTER: Juan Rodriguez-Molina
PROVIDER: E-MTAB-10820 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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