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Unraveling cell type–specific and reprogrammable human replication origin signatures associated with G-quadruplex consensus motifs

ABSTRACT: Short nascent strands purification coupled to next-generation sequencing allowed us to identify replication origins on human genome in an extensive way, by mapping replication origins in 4 different cell types, IMR-90 fibroblasts, hESC H9 cells, iPSC Th Cl-4 cells and HeLa cells. We demonstrated the existence of a cell type-specific reprogrammable signature of the cell identity revealed by specific efficiencies of conserved origin positions and not by the selection of cell-type specific subsets of origins. 4 different cell types were analyzed. For each cell types, 2 different biological replicates of short nascent strands at replication origins were purified. Each SNS sample was sequencing at least one time.

ORGANISM(S): Homo sapiens

SUBMITTER: Emilie Besnard

PROVIDER: E-GEOD-37757 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Unraveling cell type-specific and reprogrammable human replication origin signatures associated with G-quadruplex consensus motifs.

Besnard Emilie E Babled Amélie A Lapasset Laure L Milhavet Ollivier O Parrinello Hugues H Dantec Christelle C Marin Jean-Michel JM Lemaitre Jean-Marc JM

Nature structural & molecular biology 20120701 8

DNA replication is highly regulated, ensuring faithful inheritance of genetic information through each cell cycle. In metazoans, this process is initiated at many thousands of DNA replication origins whose cell type-specific distribution and usage are poorly understood. We exhaustively mapped the genome-wide location of replication origins in human cells using deep sequencing of short nascent strands and identified ten times more origin positions than we expected; most of these positions were co ...[more]

PMID: 22751019

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