Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of effects of exercise-induced Extracellular Vesicles on an F344 rat (Rattus norvegicus) model of forced running wheel exercise


ABSTRACT: n total, 16 7-week-old male F344 rats were subjected to model regular exercise and sedentary lifestyle. In the second week after arrival and acclimatization to the reversed light/dark cycle, rats were introduced to the forced running wheel without a specific running mode. It was followed by a 12-day training phase for all 16 experimental animals (started at 9 weeks of age) by gradually increasing the running speed and duration. During the training phase, 8 best performers were selected for the runner group while the remaining 8 rats were assigned to the sedentary group. The regular forced running exercise phase for the runner group lasted for 5 weeks in total, including 4 weeks of regular running. After 4 weeks of regular exercise, rats underwent brief anesthesia and of blood from the tail vein were taken. This step was repeated for the same animals immediately after 1h of running. On the same day, a blood sample was collected from the sedentary rats following the same protocol. Plasma was collected and immediately frozen for further EV isolation. EVs were isolated from rat plasma by size exclusion chromatography. RNA libraries were constructed using CleanTag® Small RNA Library Prep Kit (Trilink Biotechnologies, USA) and sequenced on Illumina NextSeq500 instrument using NextSeq 500/550 Mid Output Kit v2.5 (150 cycles).

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Rattus norvegicus

SUBMITTER: Pawel Zayakin 

PROVIDER: E-MTAB-11020 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Potential Role of Exercise Induced Extracellular Vesicles in Prostate Cancer Suppression.

Zhang Ying Y   Kim Jin-Soo JS   Wang Tian-Zhen TZ   Newton Robert U RU   Galvão Daniel A DA   Gardiner Robert A RA   Hill Michelle M MM   Taaffe Dennis R DR  

Frontiers in oncology 20210914


Physical exercise is increasingly recognized as a valuable treatment strategy in managing prostate cancer, not only enhancing supportive care but potentially influencing disease outcomes. However, there are limited studies investigating mechanisms of the tumor-suppressive effect of exercise. Recently, extracellular vesicles (EVs) have been recognized as a therapeutic target for cancer as tumor-derived EVs have the potential to promote metastatic capacity by transferring oncogenic proteins, integ  ...[more]

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