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Reconstructing the RUNX2 transcriptional hierarchy in thyroid cancer using a multi-layered network- based approach

ABSTRACT: The aim of this experiment was to identify the RUNX2-dependent transcriptional program in thyroid cancer. To this end TPC1 cell line was infected with dCas9-KRAB and sgRNA targeting RUNX2 distal promoter or a non-targeting sgRNA as control. RNA was collected 10 days after infection and changes in gene expression were evaluated by mRNA-seq. Three replicates were analyzed.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Federica Torricelli

PROVIDER: E-MTAB-11049 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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