ACGH analyses of DSB-induced LOH in Blm-deficient mESCs

ABSTRACT: Genome modification in mouse embryonic stem cells (mESCs) has provided the versatile platform to analyzing gene functions in mammalian cells. Bloom helicase-deficient mESCs showed elevated rate of loss of heterozygosity (LOH) through mitotic recombination. By utilizing this characteristic of Blm deficiency, we established an efficient method to introduce homozygous mutations into mESCs, which can be used for phenotype-driven recessive screen. It is generally thought that mitotic recombination is initiated by double-strand break. DNA repair machinery, however, possesses mechanisms that suppress deleterious crossovers between homologous chromosomes. Bloom helicase plays a central role to suppress such crossover; hence its deficiency causes elevated frequency of mitotic recombination. To dir

ORGANISM(S): Mus musculus

SUBMITTER: Kosuke Yusa 

PROVIDER: E-MTAB-1116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress