Single Cell RNA-Sequencing of RNAi treated Schneider S2 cells
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ABSTRACT: 2 million cells were seeded in a 2 ml medium containing 58 µg of total dsRNA. For coRNAi treatments 28 µg of each single dsRNA were used. After treatment cells were left to incubate 4 d at 25 °C, and plates were sealed with Parafilm to avoid medium evaporation. 8 samples were prepared with the following dsRNA treatment combinations: RLUC/RLUC x 2, CSN5/RLUC, CSN8/RLUC, CDK2/CSN5, CDK2/CSN8, CDK2/RLUC 2x . dsRNAs were taken from the HD3-dsRNA-library. Two dsRNAs per gene were pooled. The single-cell transcriptomic sequencing experiment was performed with 10x Genomics technology according to the manufacturer’s protocol.
INSTRUMENT(S): NextSeq 550
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Michael Boutros
PROVIDER: E-MTAB-11196 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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