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A high-resolution single-cell multiomic atlas of the human fetal lung - ATAC-seq

ABSTRACT: We obtained human embryonic and fetal lungs from 5-22 pcw for scRNAseq and scATACseq analysis. To focus on epithelial differentiation and region specialization, we deeply sampled 15, 18, 20 and 22 pcw lungs and separated proximal and distal regions while leaving lungs at 5, 6, 9 and 11 pcw intact. These cell samples (except for one at 6pcw) were split and processed for both scRNAseq and scATACseq.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Wei Kheng Teh

PROVIDER: E-MTAB-11266 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates.

He Peng P Lim Kyungtae K Sun Dawei D Pett Jan Patrick JP Jeng Quitz Q Polanski Krzysztof K Dong Ziqi Z Bolt Liam L Richardson Laura L Mamanova Lira L Dabrowska Monika M Wilbrey-Clark Anna A Madissoon Elo E Tuong Zewen Kelvin ZK Dann Emma E Suo Chenqu C Goh Isaac I Yoshida Masahiro M Nikolić Marko Z MZ Janes Sam M SM He Xiaoling X Barker Roger A RA Teichmann Sarah A SA Marioni John C JC Meyer Kerstin B KB Rawlins Emma L EL

Cell 20221201 25

We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific se ...[more]

PMID: 36493756

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