Dataset Information
RNA-seq to investigate effect of what shock on procyclic-form trypanosomes - ribosomal subunit
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina HiSeq 2000
ORGANISM(S): Trypanosoma brucei brucei
SUBMITTER: Christine Clayton
PROVIDER: E-MTAB-11314 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Translation Regulation and RNA Granule Formation after Heat Shock of Procyclic Form Trypanosoma brucei: Many Heat-Induced mRNAs Are also Increased during Differentiation to Mammalian-Infective Forms.
PLoS neglected tropical diseases 20160908 9
African trypanosome procyclic forms multiply in the midgut of tsetse flies, and are routinely cultured at 27°C. Heat shocks of 37°C and above result in general inhibition of translation, and severe heat shock (41°C) results in sequestration of mRNA in granules. The mRNAs that are bound by the zinc-finger protein ZC3H11, including those encoding refolding chaperones, escape heat-induced translation inhibition. At 27°C, ZC3H11 mRNA is predominantly present as an untranslated cytosolic messenger ri ...[more]
