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ChIP-seq analysis of Foxa2 binding sites in midbrain dopaminergic progenitors

ABSTRACT: Embryonic stem (ES) cells were differentiated in culture to midbrain dopaminergic (mDA) progenitors and subjected to ChIP-seq analysis to resolve genome-wide binding sites of forkhead box protein A2 (Foxa2). Foxa2 was found to directly regulate multiple lineage pathways to specify midbrain dopaminergic and floor plate progenitor identity.

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-1137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide characterization of Foxa2 targets reveals upregulation of floor plate genes and repression of ventrolateral genes in midbrain dopaminergic progenitors.

Metzakopian Emmanouil E Lin Wei W Salmon-Divon Mali M Dvinge Heidi H Andersson Elisabet E Ericson Johan J Perlmann Thomas T Whitsett Jeffrey A JA Bertone Paul P Ang Siew-Lan SL

Development (Cambridge, England) 20120613 14

The transcription factors Foxa1 and Foxa2 promote the specification of midbrain dopaminergic (mDA) neurons and the floor plate. Whether their role is direct has remained unclear as they also regulate the expression of Shh, which has similar roles. We characterized the Foxa2 cis-regulatory network by chromatin immunoprecipitation followed by high-throughput sequencing of mDA progenitors. This identified 9160 high-quality Foxa2 binding sites associated with 5409 genes, providing mechanistic insigh ...[more]

PMID: 22696295

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