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Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell transcriptomics reveals heterogeneous metabolic profiles in primary human hepatocytes

ABSTRACT: Through scRNA-sequencing of primary human hepatocytes (PHHs), we have identified four subgroups of hepatocytes. A phenotyping 5-probe cocktail (Sanofi-Aventis) has been used to assess their metabolic capacity. Upon cocktail treatment, the characterized four hepatocyte subgroups displayed differential gene expression profiles and exhibited xenobiotic metabolism-related specialization. Intracellular lipid accumulation achieved through loading the cells with free fatty acids (FFA, 2:1 oleic:palmitic acid), differently affected the four subgroups. Moreover, we have shown that intracellular fat accumulation diminishes the drug-related metabolic capacity of hepatocytes.

INSTRUMENT(S): Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1, Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Maria Richter

PROVIDER: E-MTAB-11530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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