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Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (wild type, HEI10oe, zyp1, and zyp1 HEI10oe)

ABSTRACT: This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis HEI10oe, zyp1, and zyp1 HEI10oe compared to wild type. Plants double heterozygous of zyp1-1+/- HEI10oe (Col) were crossed with zyp1-6+/- mutant plants (Ler) to generate zyp1-1/zyp1-6 HEI10oe heterozygous, HEI10oe heterozygous, zyp1-1/zyp1-6 and wild type in Col/Ler hybrid background, and then crossed as male or as female with wild-type Col. Backcross populations were grown in the greenhouse (16 h day/8 h night) for 3 weeks and 4 days in the dark, 100-150mg leave samples from plants from the 8 obtained BC1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Qichao Lian

PROVIDER: E-MTAB-11696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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