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Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (wild type and mlh1)

ABSTRACT: This experiment aims at analyzing crossover distribution in female and male meiosis, in the Arabidopsis mlh1 compared to wild type. Plants heterozygous for the mlh1-2 mutation (Col-0) were crossed as females with plants heterozygous for the mlh1-3 mutation (Ler). Wild-type and mlh1-2/mlh1-3 plants were selected among the F1s and crossed as female or as male with wild-type Col-0. Leaf samples from the four back-crossed populations were used for DNA purification and library preparation for Illumina sequencing (NextSeq 2000 2×150 base pairs [bp]), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/).

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER:

PROVIDER: E-MTAB-14422 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-12838

REPOSITORIES: biostudies-arrayexpress

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