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Identify acetylation marks in adult mouse L4 vertebrae gene promoter regions from maternal high fat diet dams using H3K27ac ChIP-Seq

ABSTRACT: Genes with increased H3K27ac marks in their promoters were found in adult mice L4 vertebrae from mice using H3K27ac ChIP-Seq. Recent data suggests that in animals and humans that a maternal diet high in fat leads to poor bone growth and development in offspring. Genes with increased H3K27ac epigenetic marks normally have higher levels of expression. Understanding which genes are being upregulated due to maternal or offspring high fat diet would allow for better treatments to combat bone loss to be developed.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Perry Caviness

PROVIDER: E-MTAB-11718 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identify acetylation marks from embryonic calvaria cell gene promoter regions from maternal high fat or control diet dams using H3K27ac ChIP-Seq.

Project description:Genes with increased H3K27ac marks in their promoters were found in mice embryonic calvaria cells using H3K27ac ChIP-Seq. Recent data suggests that in animals and humans that a maternal diet high in fat leads to poor bone growth and development in offspring. Genes with increased H3K27ac epigenetic marks normally have higher levels of expression. Understanding which genes are being upregulated in the offspring due to maternal diet would allow for better treatments to combat bone loss to be developed.

2022-05-31 | E-MTAB-11712 | biostudies-arrayexpress

Identify trimethylation marks from embryonic calvaria cell gene promoter regions from maternal high fat or control diet dams using H3K27me3 ChIP-Seq.

Project description:Genes with increased H3K27me3 marks in their promoters were found in mice embryonic calvaria cells using H3K27me3 ChIP-Seq. Recent data suggests that in animals and humans that a maternal diet high in fat leads to poor bone growth and development in offspring. Genes with increased H3K27me3 epigenetic marks normally have lower levels of expression. Understanding which genes are being downregulated in the offspring due to maternal diet would allow for better treatments to combat bone loss to be developed.

2022-05-31 | E-MTAB-11713 | biostudies-arrayexpress

DNA methylation alternations in the offspring liver from high fat diet dams

Project description:Maternal high fat diet may disturb susceptibility in offspring. liver from maternal high fat diet has CpG sites that exhibit differential DNA methylationregulated compared to control.

2019-11-28 | GSE136814 | GEO

DNA methylation alternations in the offspring liver from inulin early intervention dams

Project description:Maternal high fat diet may disturb susceptibility in offspring. liver from maternal inulin early intervention has CpG sites that exhibit differential DNA methylationregulated compared to high fat diet.

2020-01-10 | GSE136766 | GEO

DNA methylation alternations in the offspring brown adipose tissue from high fat diet dams

Project description:Maternal high fat diet may disturb susceptibility in offspring. Brown adipose tissue from maternal high fat diet has CpG sites that exhibit differential DNA methylationregulated compared to control.

2021-07-26 | GSE173218 | GEO

DNA methylation alternations in the offspring adipose from chromium restriction diet dams

Project description:Maternal chromium restriction may disturb susceptibility in offspring. Adipose from maternal chromium diet has 1214 CpG sites that exhibit differential DNA methylationregulated compared to control. We performed DNA methylation array analyses of offspring adipose from chromium restriction dams and control diet dams at 32 week (n=3 per group).

2016-09-08 | E-GEOD-82030 | biostudies-arrayexpress

Influence of generational obesity on the visceral adipose proteome

Project description:Obesity is a global health concern affecting over 650 million adults with a major contributor being an increased consumption of a high-fat diet. Maternal obesity often results in an increased risk of offspring developing obesity. In this study, we examined the effect of diet on visceral adipose in a pre-clinical model of generational diet-induced obesity that included maternal cohorts of C57BL/6 mice fed either a control diet (10% fat) or a high-fat diet (45% fat) and the resulting female offspring fed either diet. Using bottom-up proteomics on omental adipose tissue, differential protein expression was determined with the greatest difference resulting from the generational obese cohort. Differentially expressed proteins were involved in pathways related to cancer, inflammatory disease and immune response. Taken together, the results of this study provide molecular-level insight that will enable the development of more targeted, modifiable interventions that could be implemented pre-, during and post-pregnancy.

2024-10-17 | PXD042092 | Pride

Are you also what Your Mother Eats? Distinct Proteomic Portrait as a Result of Maternal High-Fat Diet in the Cerebral Cortex of the Adult Mouse.

Project description:Background: Epidemiological studies suggest an association between maternal obesity and adverse neurodevelopmental outcomes in offspring. Objective: To compare the global proteomic portrait in the cerebral cortex between mice born to mothers on a high-fat or control diet who themselves were fed a high-fat or control diet. Methods: Male mice born to dams fed a control (C) or high fat (H) diet four weeks before conception and during gestation and lactation were assigned to either C or H diet at weaning. Mice (n=24) were sacrificed at 19-weeks and their cerebral cortices were pooled into 8 samples and analysed using an iTRAQ based 2D LC-MS methodology. Results: A total of 6,695 proteins were identified and fully quantified (q<0.01). Approximately 10% of these proteins demonstrated a minimum of one Standard Deviation of regulation across all biological replicates in at least one of the experimental groups (CH, HC, HH) relative to the control (CC). Principal component analysis and hierarchical clustering analysis showed that mice clustered based on the diet of the mother and not their current diet. In silico bioinformatics analysis revealed that maternal high-fat diet was significantly associated with response to hypoxia/oxidative stress and apoptosis in the cerebral cortex of the adult offspring. Conclusion: Maternal high-fat diet was associated with distinct endophenotypic changes of the adult mouse cerebral cortex independent of the diet of the offspring. The identified modulated proteins could represent novel therapeutic targets for the prevention of neuropathological features resulting from maternal obesity.

2018-01-29 | PXD001540 | Pride

Maternal Nutrition Induces Pervasive Gene Expression Changes but no Detectable DNA Methylation Differences in the Liver of Adult Offspring [RRBS]

Project description:Aims: Epidemiological and animal studies have shown that maternal diet can influence metabolism in adult offspring. However, the molecular mechanisms underlying these changes remain poorly understood. Here, we aim to explore phenotypes induced by maternal obesity in a mouse model and examine gene expression and epigenetic alterations in adulthood induced by maternal diet. Methods: We analyzed genetically identical male mice born from dams fed a high- or low-fat diet throughout pregnancy and until day 21 postpartum. After weaning, half of the males of each group were fed a high-fat diet, the other half a low-fat diet. We first characterized the genome-wide gene expression patterns of six tissues of adult offspring - liver, pancreas, white adipose, brain, muscle and heart [GSE40903] . We then measured DNA methylation patterns in liver at selected loci and throughout the genome. Results: Maternal diet had a significant effect on the body weight of the offspring when they are fed an obesogenic diet after weaning. Our analyses showed that maternal diet had a pervasive effect on gene expression, with a pronounced effect in liver where it affected many genes involved in inflammation, cholesterol synthesis and RXR activation. Maternal diet had no detectable effect on DNA methylation in the liver. Conclusions: Overall, our findings highlighted the persistent influence of maternal diet on adult tissue regulation and suggested that the transcriptional changes were unlikely to be caused by DNA methylation differences in adult liver. Methylation is compared between nine week old animals fed a common diet as adults, but derived from mothers fed different diets.

2014-03-04 | E-GEOD-52266 | biostudies-arrayexpress

DNA methylation alternations in the offspring liver from chromium restriction diet dams

Project description:Maternal chromium restriction may disturb susceptibility in offspring. Adipose from maternal chromium diet has 935 genes that exhibit differential DNA methylationregulated compared to control.

2019-05-30 | GSE82029 | GEO

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