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Transcriptional profiling of CD133+/high vs CD133-/low cell populations of Caco2, HT-29 and HUH7 cell lines

ABSTRACT: Transcriptional profiling of CD133+/high vs CD133-/low cell populations of Caco2, HT-29 and HUH7 cell lines. Further in silico analysis of differentially expressed transcripts and proteins in CD133+/high vs CD133-/low cell populations revealed several potential key molecular regulators of CD133. In silico data was proved by CRISPR/CAS9 gene editing system.

ORGANISM(S): Homo sapiens

SUBMITTER: Yan Kim

PROVIDER: E-MTAB-11779 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TRIM28 Is a Novel Regulator of CD133 Expression Associated with Cancer Stem Cell Phenotype.

Kim Yan S YS Potashnikova Daria M DM Gisina Alisa M AM Kholodenko Irina V IV Kopylov Arthur T AT Tikhonova Olga V OV Kurbatov Leonid K LK Saidova Aleena A AA Tvorogova Anna V AV Kholodenko Roman V RV Belousov Pavel V PV Vorobjev Ivan A IA Zgoda Victor G VG Yarygin Konstantin N KN Lupatov Alexey Yu AY

International journal of molecular sciences 20220830 17

CD133 is an extensively studied marker of the most malignant tumor cell population, designated as cancer stem cells (CSCs). However, the function of this glycoprotein and its involvement in cell regulatory cascades are still poorly understood. Here we show a positive correlation between the level of CD133 plasma membrane expression and the proliferative activity of cells of the Caco-2, HT-29, and HUH7 cancer cell lines. Despite a substantial difference in the proliferative activities of cell pop ...[more]

PMID: 36077272

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