Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

36 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole Transcriptome Microarray of Primary Human Monocytes Transfected With MiR-125a

ABSTRACT: Conducted to obtain information of the regulatory impact of miR-125a in human primary monocytes.

ORGANISM(S): Homo sapiens

SUBMITTER: Max Hübner

PROVIDER: E-MTAB-11824 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Whole transcriptome microarray of U87 Gliblastoma cells transfected with mir-93 or negative Control and stimulated with IL1b

Project description:We inended to investigate the regulatory impact of miR-93 on Glioblastoma cells during an inflammatory Stimulus with a special focus on pro-inflammatory pathways and cytokines.

2020-04-29 | E-MTAB-8395 | biostudies-arrayexpress

CodeLink Mouse Whole Genome Bioarray data from hematopoietic stem cells (HSCs) of miR-125a (+/+) wild-type and miR-125a knockout mice with myeloproliferative disorder (MPD)

Project description:miR-125a knockout mice develop myeloproliferative disorder (MPD). To investigate the molecular mechanisms of MPD induced by the loss of miR-125a, gene expression profiling on hematopoietic stem cells of miR-125a (+/+), (+/-) MPD, and (-/-) MPD mice was performed by using CodeLinkTM Whole Genome DNA array analysis. CodeLink Mouse Whole Genome Bioarrays were performed according to the manufacturerâs instruction on total RNA extracted from hematopoietic stem cells of miR-125a (+/+) wild-type mouse, miR-125a (+/-) heterozygous mouse with MPD, and miR-125a (-/-) homozygous mouse with MPD. One replicate per array.

2015-12-31 | E-GEOD-69621 | biostudies-arrayexpress

CodeLink™ Mouse Whole Genome Bioarray data from hematopoietic stem cells (HSCs) of miR-125a (+/+) wild-type mice and miR-125a (+/-) heterozygous mice with myeloproliferative disorder

Project description:miR-125a knockout mice develop myeloproliferave disorder (MPD). To investigate the molecular mechanisms of MPD induced by the loss of miR-125a, gene expression profiling on hematopoietic stem cells of miR-125a (+/+) and (+/-) MPD mice was performed using CodeLink Whole Genome DNA array analysis. Codelink Mouse Whole Genome Bioarrays were performed according to the manufacturer’s instruction on total RNA extracted from hematopoietic stem cells of miR-125a (+/+) wild-type mice and miR-125a (+/-) heterozygous mice with MPD. One replicate per array.

2015-06-30 | E-GEOD-65648 | biostudies-arrayexpress

Expression profiles of HSPCs overexpressing microRNA cluster 99b/let-7e/125a, miR-125a, miR-155 and empty vector.

Project description:Hematopoietic stem/progenitor cell (HSPC) traits differ between genetically distinct mouse strains. For example, DBA/2 mice have a higher HSPC frequency compared to C57BL/6 mice. We performed a genetic screen for microRNAs that are differentially expressed between LSK, LS−K+, erythroid and myeloid cells isolated from C57BL/6 and DBA/2 mice. This analysis identified 131 microRNAs that were differentially expressed between cell types and 15 that were differentially expressed between mouse strains. Of special interest was an evolutionary conserved miR-cluster located on chromosome 17 consisting of miR-99b, let-7e and miR-125a. All cluster members were most highly expressed in LSKs and down-regulated upon differentiation. In addition, these microRNAs were higher expressed in DBA/2 cells compared to C57BL/6 cells, and thus correlated with HSPC frequency. To functionally characterize these microRNAs, we overexpressed the entire miR-cluster 99b/let-7e/125a and miR-125a alone in BM cells from C57BL/6 mice. Overexpression of the miR-cluster or miR-125a dramatically increased day-35 CAFC activity and caused severe hematopoietic phenotypes upon transplantation. We showed that a single member of the miR-cluster, namely miR-125a, is responsible for the majority of the observed miR-cluster overexpression effects. Finally, we performed genome-wide gene expression arrays and identified candidate target genes through which miR-125a may modulate HSPC fate. These data consist of total mRNA obtained from 7-day cultured post 5-FU BM cells positively transduced with empty vector, miR-cluster 99b/let-7e/125a, miR-125a or miR-155 retroviral vector. Dataset (A) and dataset (B) were analyzed individualy. In each dataset, all samples were analyzed in independent biological triplicates.

2011-12-15 | E-GEOD-33689 | biostudies-arrayexpress

Genetic Screen Identifies MicroRNA Cluster 99b/let-7e/125a as a Regulator of Primitive Hematopoietic Cells

Project description:This SuperSeries is composed of the following subset Series: GSE33689: Expression profiles of HSPCs overexpressing microRNA cluster 99b/let-7e/125a, miR-125a, miR-155 and empty vector. GSE33690: MicroRNA profiles of four developmentally related hematopoietic cell types isolated from the BM of B6 and D2 mice. Refer to individual Series

2011-12-15 | E-GEOD-33691 | biostudies-arrayexpress

Stress response of Cupriavidus metallidurans strains (AE104 delta 7)

Project description:Response of Cupriavidus metallidurans AE104(delta zupT) mutant to Zn/EDTA stress In this study 2 different treatments were performed to acquire expression profiles of the total genome of Cupriavidus metallidurans

2015-08-01 | E-GEOD-71011 | biostudies-arrayexpress

Response of Cupriavidus metallidurans CH34 to different Pt-species

Project description:Response of Cupriavidus metallidurans CH34 to cisplatin, Pt(IV)chloride and Au-NP In this study 7 different treatments were performed (first 2 as 3 replicates) to acquire expression profiles of the total genome of Cupriavidus metallidurans

2016-02-27 | E-GEOD-78723 | biostudies-arrayexpress

Stress response of Cupriavidus metallidurans strains

Project description:Response of Cupriavidus metallidurans CH34, AE104 and delta zupT mutant to Zn/EDTA stress In this study 3 different treatments were performed to acquire expression profiles of the total genome of Cupriavidus metallidurans

2014-12-16 | E-GEOD-64196 | biostudies-arrayexpress

Response of Cupriavidus metallidurans CH34 to different Au-species

Project description:Response of Cupriavidus metallidurans CH34 to NaAuCl4, Au(I)-thiomalate, Au(I)-thiosulfate, KAuCN, (KCN as control) In this study 10 different treatments were performed (first 7 as 3 replicates) to acquire expression profiles of the total genome of Cupriavidus metallidurans

2013-03-15 | E-GEOD-42199 | biostudies-arrayexpress

miR-125a Confers Multi-Lineage Long-Term Repopulating Stem Cell Activity to Murine and Human Hematopoietic Progenitors

Project description:Umbilical cord blood (CB) is a non-invasive, convenient and broadly used source of hematopoietic stem cells (HSCs) for allogeneic stem cell transplantation. However, limiting numbers of HSCs remain a major constraint for its clinical application. One feasible option would be to expand HSCs to improve therapeutic outcome, however available protocols and the molecular mechanisms governing the self-renewal of HSC are unclear. Here we show that ectopic expression of a single miRNA, miR-125a, in purified murine and human multipotent progenitors (MPP) resulted in increased self-renewal and robust long-term multi-lineage repopulation in transplanted recipient mice. Using quantitative proteomics and Western blot analysis, we identified a restricted set of miR-125a targets which revealed the involvement of the MAP kinase signaling pathway in conferring long-term repopulating capacity to multipotent progenitors in human and mice. Our findings offer the innovative potential to use MPP with enhanced self-renewal activity to augment limited sources of HSC to improve clinical protocols.

2017-01-11 | PXD003931 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data