Unknown,Transcriptomics,Genomics,Proteomics

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Identification of HCC-specific genes in a rat model of hepatocarcinogenesis


ABSTRACT: The diethylnitrosamine HCC rat model (DEN-HCC) is a useful preclinical model mirroring human hepatocellular carcinoma. To overcome tumor heterogeneity of human HCCs linked to different etiologies and clonal selection, rat HCC samples were compared with matched non-tumor livers in order to identify HCC-related genes involved in the carcinogenetic process. Specifically, male Wistar rats received DEN (Sigma-Aldrich) in the drinking water (100 mg/L) for 8 weeks. Two weeks later the end of DEN treatment, animals were monitored by ultrasound imaging. Animals were euthanized 1 or 2 months after the end of DEN treatment, when the major diameter of at least one HCC nodule reached a dimension of 10 mm. Samples were collected for molecular biology and total RNA for microarray analysis was extracted by using Trizol. Two rat livers from untreated mice were included in the analysis.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Cristian Bassi 

PROVIDER: E-MTAB-11977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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