Transcriptome profiling by RNA-seq of the Pseudomonas aeruginosa strain PA14 deleted for the small RNA GssA compared with PA14 wild-type

ABSTRACT: To obtain a preliminary picture at the transcriptional level of the set of genes linked to the regulatory activity of the small RNA GssA of P. aeruginosa PA14, we analyzed the global transcription profile of PA14deltagssA strain in comparison with PA14 by an RNA-seq approach, extracting total RNA at OD600 of 2, corresponding to early stationary phase, when the two strains were grown in Brain Heart Infusion (BHI) rich medium, a condition in which, at the time of its identification, a relevant expression of GssA was noted. For each strain, three independent biological replicates were performed. The concentration and the purity of the RNA starting material were determined spectrophotometrically while RNA Integrity Number (RIN) was measured on an Agilent TapeStation 4200. An additional DNase s

INSTRUMENT(S): Illumina iSeq 100

ORGANISM(S): Pseudomonas aeruginosa UCBPP-PA14

SUBMITTER:  

PROVIDER: E-MTAB-12073 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP140193

REPOSITORIES: biostudies-arrayexpress