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Chromatin associated RNAs

ABSTRACT: RNA has been suggested to play a major role in the regulation of higher-order structures of chromatin. This study investigated chromatin-associated RNA (caRNA) in Drosophila melanogaster by applying high-throughput sequencing to RNA molecules that co-fractionate with chromatin. Using a cell-free in vitro chromatin assembly system (DREX), we demonstrated that caRNAs mainly comprise snoRNAs, snoRNA hosts and miRNAs.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sarah Diermeier

PROVIDER: E-MTAB-1237 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deep sequencing of small chromatin-associated RNA: isolation and library preparation.

Diermeier Sarah Daniela SD Schubert Thomas T Längst Gernot G

Methods in molecular biology (Clifton, N.J.) 20140101

Chromatin-associated RNA (caRNA) is a newly identified class of RNA molecules stably associated with chromatin, maintaining the higher order structure of euchromatic regions in an accessible form (Schubert et al., Mol Cell, doi:10.1016/j.molcel.2012.08.021, 2012). This section provides a detailed protocol describing the isolation of small caRNA from Drosophila cells and preparation of libraries suited for stranded small (20-200 bp) RNA deep sequencing on the Illumina platform. ...[more]

PMID: 24163001

Publication: 1/3

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