Differential RNA-Seq of Corynebacterium glutamicum wildtype, deletion mutant sigE and deletion mutant cseE reveals overlapping SigH and SigE sigma factor regulons.
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ABSTRACT: Total RNA was isolated from 3 biological replicates of C. glutamicum wildtype, deletion mutant sigE and deletion mutant cseE cells by a Quick-RNA Miniprep Plus kit (Zymo Research). The samples were treated with DNase (Roche Diagnostics) and RNA was purified with an RNA Clean&Concentrator-5 kit (Zymo Research). Ribosomal rRNA was removed with a Ribo-Zero rRNA Removal Kit for bacteria (Illumina). The purity of RNA was then tested with an Agilent RNA Pico 6000 kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). TruSeq Stranded mRNA Sample Preparation guide (Illumina) was then used to construct the cDNA library. The constructed cDNA library was then sequenced with Illumina HiSeq 1500 using a read length of 74 bases.
INSTRUMENT(S): Illumina HiSeq 1500
ORGANISM(S): Corynebacterium glutamicum
SUBMITTER:
PROVIDER: E-MTAB-12457 | biostudies-arrayexpress |
SECONDARY ACCESSION(S): E-MTAB-12458
REPOSITORIES: biostudies-arrayexpress
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