Transcriptomics

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RNA-seqs of SRSF3 and hnRNPK depleted C2C12 cells


ABSTRACT: Total RNA was extracted from C2C12 cells transfected with the indicated siRNA using TRIZOL (Thermo Fisher Scientific), and was further purified with Quick-RNA Miniprep Kit (Zymo research) according to the manufacturer’s instructions. The extracted RNA was subjected to RNA-seq at Macrogen, Japan. Briefly, a sequencing library was prepared using the TruSeq Stranded mRNA kit (Illumina), and the library was read on Illumina NovaSeq 6000 (150 bp paired-end reads).

ORGANISM(S): Mus musculus

PROVIDER: GSE161601 | GEO | 2021/07/20

REPOSITORIES: GEO

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