Unknown,Transcriptomics,Genomics,Proteomics

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Whole-genome resequencing of 90 tetrads of EMS-treated fission yeast Schizosaccharomyces pombe


ABSTRACT: This experiment aims at analyzing crossover distribution genome-wise, in the fission yeast. S. pombe strains PR109 (h- leu1-32 ura4-D18) and PR110 (h+ leu1-32 ura4-D18) were used for three successive rounds of mutagenesis with Ethylmethane Sulfonate Mutagenesis. Five independent clones of the first round of mutagenesis were at the root of two subsequent similar rounds of mutagenesis. Each clone used was checked for its ability to mate and sporulate. Eventually, five mutagenized clones from each of the PR109 and PR110 backgrounds were sequenced to identify de novo mutations and determine the optimal combinations of mutation patterns for recombination analyses. The following h- x h+ crosses were selected and the corresponding tetrads dissected: BLP49 (A) x BLP23 (I), 29 tetrads; BLP59 (C) x BLP19 (H), 30 tetrads and BLP64 (D) x BLP33 (K), 33 tetrads.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Qichao Lian 

PROVIDER: E-MTAB-12516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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