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Effects of upf1delta on gene expression

ABSTRACT: The Upf1 RNA-binding protein is essential for nonsense-mediated decay (NMD). We compared wild type (ura4-D18 leu1-32 h-) and upfdelta (upf1delta::kanMX6 ura4-D18 leu1-32 h-) cells transcriptome under normal growth conditions

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata

PROVIDER: E-MTAB-931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional characterization of Upf1 targets in Schizosaccharomyces pombe.

Matia-González Ana M AM Hasan Ayesha A Moe Gøril H GH Mata Juan J Rodríguez-Gabriel Miguel A MA

RNA biology 20130416 6

Nonsense-mediated mRNA decay (NMD) is a highly conserved mechanism of mRNA degradation. NMD eliminates mRNAs containing premature termination codons (PTCs), preventing the production of truncated proteins with possible deleterious effects. However, there is mounting evidence that NMD factors, like Upf1, Upf2 and Upf3, participate in general regulation of gene expression, affecting the expression of genes lacking PTCs. We have used the fission yeast Schizosaccharomyces pombe to identify mRNAs dir ...[more]

PMID: 23619768

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