Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional reprogramming of methionine restricted cells


ABSTRACT: The genome-wide transcriptional response of S. cerevisiae cells upon transfer to methionine-restricted media is investigated. DHO strain (BY4742 background) was cultivated batch-wise in SDC media at 30C and 180 rpm up to mid-exponential phase (OD600 ~ 0.6). The cells of the main culture were divided in three aliquots at the mid-exponential phase, centrifuged at 6000 rpm for 5 min, washed twice with deionized and distilled sterile water prior to their transfer to the treatment media. Treatment media comprised of SDC + 0.75% methionine for the methionine-restricted case while SDC is used for the control case. Sample collection for transcriptional profiling was done at the 2nd hour of the transfer. The experiments were carried out in biological triplicates.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Esra Börklü 

PROVIDER: E-MTAB-12860 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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