Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional response of S. cerevisiae to metformin treatment in carbon-limited medium

ABSTRACT: The genome-wide transcriptional response of S. cerevisiae cells upon transfer to carbon-limited or carbon-limited and metformin supplemented media is investigated. DHO strain (BY4742 background) was cultivated batch-wise in SDC media at 30C and 180 rpm up to mid-exponential phase (OD600 ~ 0.6). The cells of the main culture were divided in three aliquots at the mid-exponential phase, centrifuged at 6000 rpm for 5 min, washed twice with deionized and distilled sterile water prior to their transfer to the treatment media. Treatment media comprised of SDC + 0.25% glucose for the C-lim control case and SDC +0.25%glucose + 1.66% metformin for the Met case. Control data for the reference condition (glucose limitation) is derived from E-MTAB-3001. Sample collection for transcriptional profiling was done at the 2nd hour of the transfer. The experiments were carried out in biological triplicates.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Esra Börklü-Yücel

PROVIDER: E-MTAB-6847 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional response of S. cerevisiae to metformin treatment in carbon-limited medium

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