Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) ) on the transcriptome of CD44-overexpressing IMR90 cells.
Project description:The role of CD44 in naked-mole rats (NMR) oligodendrocyte progenitor cells (OPCs) were evaluated by comparing the transcriptome of control and CD44-knockdown NMR OPCs.
Project description:To identify the sequences responsible for recruitment of Glucocorticoid receptor (GR) to individual loci, we performed ChIP-seq and ChIP-exo that combines chromatin immunoprecipitation with an exonuclease digestion step. We performed these experiments in three cell lines : IMR90 (ATTC:CCL-186), U2OS osteosarcoma cell lines, K562 (ATCC:CCL243), upon glucocorticoid treatment.
Project description:Transcription profiling of IMR90 fibroblasts after ionizing radiation. Cells were harvested 30 minutes (acute response) or 5 days (cell cycle arrested) after being irradiated with 5Gy of ionizing radiation.
Project description:Transcriptional profiling of siRNA-mediated inhibition of CDC7 in IMR90 primary diploid fibroblasts derived from embryonic lung tissue.
Project description:Total RNA was isolated from proliferating and senescent IMR90 cells to compare gene-expression to the changes in nucleolus-association in proliferating and senescent IMR90 cells.