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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

ABSTRACT: The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

PROVIDER: PRJEB37814 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4027230_1.fastq.gz	Fastqsanger.gz
	ERR4027230_2.fastq.gz	Fastqsanger.gz
	ERR4027231_1.fastq.gz	Fastqsanger.gz
	ERR4027231_2.fastq.gz	Fastqsanger.gz
	ERR4027223_1.fastq.gz	Fastqsanger.gz

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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) ) on the transcriptome of CD44-overexpressing IMR90 cells.

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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

Project description:The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on IMR90 transcriptome

Project description:Naked mole-rat skin fibroblasts (NSF) produces vHMM-HA. Although NSF-HA has been shown to suppress NSF transformation, it is still unclear if exceptional polymer length of vHMM-HA is important for its functions. Here, we compared the effect of intact control NSF-HA (cNSF-HA) and partially fragmented NSF-HA (fNSF-HA) on the transcriptome of IMR90 cells. Related RNA-Seq datasets are deposited in ArrayExpress (E-MTAB-8943).

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The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44 protein-protein interactions.

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Project description:The effect of very-high-molecular-mass hyaluronan (vHMM-HA) on CD44-overexpressing IMR90 cells

2021-03-22 | E-MTAB-8943 | ExpressionAtlas

Expression data from CD44-knockdown MDA-MB-231 human breast cancer cells.

Project description:CD44, an adhesion molecule that binds to extracellular matrix, primarily to hyaluronan (HA), has been implicated in cancer cell migration, invasion, and metastasis. CD44 has also recently been recognized as a marker for stem cells of several types of cancer. However, the roles of CD44 in the development of bone metastasis still remain unclear. To explore this issue, we established the MDA-MB-231 human breast cancer cells stably expressing short hairpin RNA against CD44. The CD44-knockdown MDA-MB-231 cells (MDA-MB-231 shCD44-2 and shCD44-3) were analyzed. As control, MDA-MB-231 cells stably expressing shRNA against firefly luciferase (shLuc) were used. Total of three samples. No replicates.

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