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ScRNA-seq of canalicular stage human fetal lung cultured under air-liquid interface

ABSTRACT: Small pieces (<0.5 cm of diameter) were isolated from distal regions of 125 day male human fetal lung and cultured on floating poly-carbonate membranes on top of serum and growth factor free medium under air-liquid interface conditons (ALI). ALI explants underwent changes in gene expression indicative of alveolar epithelial cell differentiation under these conditions. scRNA-sequencing was performed on fetal tissue prior to culture (day 0) and day 3, 6, 9 and 12 of ALI explant culture.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Tristan Frum

PROVIDER: E-MTAB-12959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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