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RNA-seq comparison of bone marrow stem cells isolated from two genotypes WT and Ppm1dT/+

ABSTRACT: Ppm1d T/+ mice exhibit altered BM composition. The aim is to determine the molecular mechanisms possibly responsible for this alterations in sorted long-term hematopoietic stem cells isolated from bone marrow. Long-term HSCs (LT-HSCs sorted from 12-week-old WT and Ppm1DT/+ mice. The Ppm1dT/+ HSC transcriptome shows enrichment of MYC, mTOR compared to WT.

INSTRUMENT(S): RNAeasy Micro Kit (Qiagen) and KAPA mRNA HyperPrep Kit (Kapa Biosystems), KAPA mRNA HyperPrep Kits,Illumina, NextSeq 500, Influx instrument (BD Biosciences)

ORGANISM(S): Mus musculus

SUBMITTER: Rajendra Kumar Labala

PROVIDER: E-MTAB-13090 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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