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RNA-seq comparison of leukemic bone marrow (BM) cells isolated from WT, Ppm1d T/+ and healthy control

ABSTRACT: Ppm1d T/+ mice exhibit altered BM composition. The aim is to determine the molecular mechanisms possibly responsible for this alterations. Thus, leukemic mice, defined by expression of ckit+, were sorted from leukemic WT and Ppm1d T/+ mice and healthy WT and subjected to bulkRNAseq. We found that Ppm1dT/+ AML (Zbed3, Runx3, Marcks, Extl3, Pcbp4, Igf2bp3, Plch1, and Gdf6) genes were associated with AML and cancer progression.

INSTRUMENT(S): RNAeasy Micro Kit (Qiagen) and KAPA mRNA HyperPrep Kit (Kapa Biosystems), NextSeq 550, KAPA mRNA HyperPrep Kits,Illumina, Influx instrument (BD Biosciences)

ORGANISM(S): Mus musculus

SUBMITTER: Rajendra Labala

PROVIDER: E-MTAB-13056 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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