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ChIP-nexus analysis of fission yeast transcription factors Cbf11, Cbf11DBM, Cbf12, Cbf12DBM, Mga2 and Fkh2

ABSTRACT: Genome-wide identification of transcription factor (TF) binding sites in the genome of the fission yeast Schizosaccharomyces pombe. The ChIP-nexus method was used. TFs included were: Cbf11-TAP and Cbf12-TAP (and their DBM mutants with impaired DNA binding), TAP-Mga2, and Fkh2-TAP (as an irrelevant control TF). IPs from an untagged WT strain were also analyzed. Cbf11-related IPs were performed from exponential cultures, while Cbf12-related IPs were performed from stationary cultures. YES complex medium was used for all cultivations.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Martin Převorovský

PROVIDER: E-MTAB-13258 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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