Project description:Bone marrow derived phagocytes from mouse, rat, rabbit and pig stimulated with LPS (100ng/mL) or with dsRNA (poly I:C) (1ug/mL) for 0,2,4,6 hours. Processed files include UMI matrices of QCed cells that belong to the first cluster (as described in the publication), which were used for all the analyses presented in the publication.

2018-07-16 | E-MTAB-6754 | biostudies-arrayexpress

Single cell RNA-seq atlas of human NSCLC lesions and non-involved tissue

Project description:We performed single cell RNA sequencing (scRNA-seq) of NSCLC tumours and matched, adjacent, non-involved lung tissue from 24 patients. The data set is composed of approximately 900,000 cells from two different populations: CD235- (haematopoietic and non-haematopoietic cells depleted of erythrocytes), and CD45+ (all haematopoietic cells).

2024-04-29 | E-MTAB-13526 | biostudies-arrayexpress

Immune cells direct epithelial maturation during early human lung development

Project description:The adult human lung harbours a significant proportion of immune cells, which are known to play a crucial role in both normal lung homeostasis and pathogenesis. In this work, we collected CD45-enriched human lung cells from 6 to 22 week post conception (pcw) and built a single-cell transcriptome and proteome atlas. We showed a biphasic mode of immune cell development peaking at 8-9 and 20 pcw respectively. The developing immune cells potentially modulate the maturation of the epithelium through signalling molecules. We demonstrated using the lung organoid model that IL-1ß was required for epithelium differentiation. Overall, we provide a resource for the community of lung biologists and immunologists and provide insights on the interaction between the innate immune system and epithelium during fetal development.

2022-12-09 | E-MTAB-11528 | biostudies-arrayexpress

scRNA-seq of first and second trimester human gonads - 10x dataset

Project description:scRNA-seq of first and second trimester human gonads

2022-06-30 | E-MTAB-10551 | biostudies-arrayexpress