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Unknown,Transcriptomics,Genomics,Proteomics

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Mapping the functional impact of non-coding regulatory elements in primary T cells through single-cell CRISPR screens

ABSTRACT: The vast majority of complex disease associations cannot be cleanly mapped to a gene, as they often lie within the non-cding fraction of the genome. Immune disease-associated variants are enriched within regulatory elements, such as distal enhancers, found in T cell-specific open chromatin regions. To identify the genes modulated by these regulatory elements, we developed a CRISPRi-based single-cell functional screening approach in primary human CD4+ T cells. We performed a proof-of-concept screen targeting 45 non-coding regulatory elements and 35 transcription start sites, each targeted by 4 different gRNAs. We profiled approximately 250,000 CD4+ T cell single-cell transcriptomes using 3' 10X Genomics.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Ximena Ibarra-Soria

PROVIDER: E-MTAB-13324 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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