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ChIP-seq of RNA Polymerase II (Rpb3) from wild-type and Rpb7-QHF cells

ABSTRACT: Our structural and biochemical studies show that S. cerevisiae RNA Polymerase II (RNAPII) homodimerizes through the stalk domain (formed by the Rpb4-Rpb7 subunits). To explore the biological impact of disrupting this interaction we introduced a triple point mutation at the RNAPII dimerization interface in Rpb7 (Q96A, H97A, F109K). To test the impact of the dimerziation mutant on RNAPII occupancy, we performed ChIP-seq using a monoclonal antibody against the Rpb3 subunit (1Y26, abcam) of RNAPII, in WT and Rpb7-QHF cells.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Saccharomyces cerevisiae S288C

SUBMITTER: Juan Rodriguez-Molina

PROVIDER: E-MTAB-13369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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