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Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli

ABSTRACT: To identify YpdB-regulated genes, the transcriptome profiles of E. coli cells overproducing either the response regulator (RR) YpdB or the RR YehS (control) were comparatively analyzed. The expression level of 15 genes varied more than 1.9-fold.

ORGANISM(S): Escherichia coli

SUBMITTER: Luitpold Fried

PROVIDER: E-MTAB-1347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of a target gene and activating stimulus for the YpdA/YpdB histidine kinase/response regulator system in Escherichia coli.

Fried Luitpold L Behr Stefan S Jung Kirsten K

Journal of bacteriology 20121207 4

Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overproducing either YpdB or a control protein. Expression levels of 15 genes differed by more than 1.9-fold between the two strains. A comprehensive evaluation of these genes identified ...[more]

PMID: 23222720

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