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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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10X genomics libraries for scRNA-seq of zebrafish embryos (2dpf) treated with uncoated and oligolysine-PEG copolymer coated DNA origami wireframe nanosheets

ABSTRACT: To study the biodistribution of DNA origami in zebrafish and to determine differences in biodistribution based on the coating of the nanostructure using oligolysine-PEG copolymers, zebrafish embryos (2dpf) were injected with coated or uncoated DNA origami (wireframe nanosheets) without any targeting molecule, but containing a detection fluorophores. After treatment, the embryos were disassociated into a single-cell suspension and sorted through FACS for embryos containing nanostructures (fluorophores). 10X sc-libraries were generated from the sorted embryos and sequenced to establish cell type predictions for cells with coated and uncoated structures respectively.

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Danio rerio

SUBMITTER: Enya Engström

PROVIDER: E-MTAB-13554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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