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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA sequencing of mouse myeloid cardiac tdTomato+ and tdTomato- cells (Fgd5-CreERT2) post-myocardial infarction upon DMSO and 4-oxo-RA treatment.

ABSTRACT: This lineage tracing experiment focused on studying the transcriptome of cardiac myeloid cells derived from BM HSCs post-MI. We performed Left coronary artery ligation (LAD) in a tamoxifen-inducible in vivo HSC-lineage tracing mouse model which allows HSC-progeny lineage tracing via Tomato expression (Fgd5-CreERT2 tdTomato; PMID: 30561324). This was followed by a 2-day treatment with either vehicle (DMSO) or 4-oxo-RA. On day 3 post-MI, hearts were extracted, and tdTomato-positive and tdTomato-negative myeloid cells (CD11b+) were isolated from the myocardium. scRNA-seq was performed.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-MTAB-13580 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP166885

REPOSITORIES: biostudies-arrayexpress

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