Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of mock oligodendrocytes (Ols) and tdTomato+ and tdTomato- Ols Transcriptomes.

ABSTRACT: Purpose: Comparision transcriptomes of isolated tdTomato+ and tdTomato- OLs from the brains and spinal cords of infected mice at 30 dpi to those of OLs isolated from mock-infected central nervous system (CNS) samples by nextgen sequencing Methods: Brains and spinal cords were harvested at day 30 p.i. and processed as described above. After Live/Dead staining and treatment with Fc block, OLs were identified using APC-anti-O4 (1:50; Miltenyi). RNA was extracted from sorted OLs using an RNeasy Micro Kit (Qiagen). RNA sequence analysis was performed at the University of Minnesota Genomics Center. Results: We identified 158 and 115 genes, that showed a significant difference (p<0.05) when tdTomato+ and tdTomato- cells were compared to cells from mock-infected mice, respectively, with 61 mRNAs common to both tdTomato- and tdTomato+ cells

ORGANISM(S): Mus musculus

PROVIDER: GSE148650 | GEO | 2020/04/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of mock oligodendrocytes (Ols) and tdTomato+ and tdTomato- Ols Transcriptomes.

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of mock oligodendrocytes (Ols) and tdTomato+ and tdTomato- Ols Transcriptomes.

| PRJNA625280 | ENA

scRNA-seq to examine the reaction of a subpopulation of ependymal cells, EpA cells, to spinal cord injury.

Project description:We performed single cell RNA sequencing to examine the reaction of a subpopulation of ependymal cells, EpA cells, to spinal cord injury. The experiment contains cells from spinal cords of Troy-CreERT2 mice on a Rosa26-tdTomato background. The spinal cord samples come from uninjured and injured spinal cords (3 days after injury).

2022-01-03 | GSE192824 | GEO

Transcription profiling of mouse spinal cord from mock-infected and Theilers murine encephalomyelitis (TME)-virus infected animals - time series

Project description:Theilers murine encephalomyelitis (TME) is an experimentally virus-induced demyelinating leukomyelitis, displaying clinical and pathological similarities to chronic progressive multiple sclerosis. <br>The aim of this study was to identify pathways associated with demyelination using an assumption-free microarray approach. <br>Five-week-old female SJL/JHanHsd-mice were intracerebrally infected with the BeAn-strain of the TME- virus (TMEV) or mock-infected with vehicle only.<br>Groups of 5-6 TMEV- and Mock-infected mice were killed at 14, 42, 98, and 196 days post infection.<br>Total RNA was isolated from the spinal cords and gene expression was measured employing Affymetrix mouse genome 430 2.0 arrays.

2009-06-19 | E-MEXP-1717 | biostudies-arrayexpress

CLIPseq of hnRNP A1 from the spinal cords of mice with experimental autoimmune encephalomyelitis

Project description:Experimental autoimmune encephalomyelitis (EAE) is a mouse model for multiple sclerosis (MS) a chronic autoimmune disease of the central nervous system. We have observed dysfunction of the RNA binding protein hnRNP A1 in neurons from the brains of patients with MS, and the spinal cords of mice with EAE. Here, we sought to characterize the consequences of EAE-induced dysfunction of hnRNP A1 on the RNAs it binds by using CLIPseq to establish both the normal central nervous system RNA binding profile of hnRNP A1 in the spinal cords of naive mice, and any alterations to the binding profile of hnRNP A1 in the spinal cords of mice with EAE.

2024-01-04 | GSE208093 | GEO

Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and inhibitory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031535 | Pride

Global proteome of Atg5flox:CamKIIα-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain excitatory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:CamKIIα-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:CamKIIα-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and excitatory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031552 | Pride

Genome-wide expression analysis of human fibroblasts and converted neurons

Project description:Human fibroblasts can be directly converted into cholinergic neurons by Neurogenin 2 (Neurog2 or NGN2) under the treatments of small molecules. Genome-wide analysis of gene expression was performed to examine the similarity of converted neurons to samples from human brain or spinal cord. Total RNA obtained from isolated human fetal lung fibroblasts or converted neurons at 21 days. Commercially available total RNAs from adult human brains and spinal cords were used as controls.

2014-01-16 | E-GEOD-45954 | biostudies-arrayexpress

N39 APOA1-tdTomato drug screen

Project description:N39 APOA1-tdTomato drug screen

| PRJNA1142560 | ENA

scRNAseq from spinal cords

Project description:scRNAseq from spinal cords

| PRJNA704325 | ENA

CUT&RUN-seq of DPPA2 in Esg1-tdTomato reporter mESC line

Project description:Enrichment of DPPA2 was assessed by CUT&RUN-sequencing in wildtype Esg1-tdTomato reporter mESC line.

2021-11-23 | E-MTAB-11182 | biostudies-arrayexpress